芪葵顆粒對糖尿病腎病大鼠腎臟中TGF—β1及MCP—1表達的影響

楊晨+朱萱萱++余江毅++張迪

[摘要] 目的 觀察芪葵顆粒對糖尿病腎病大鼠腎臟中轉(zhuǎn)化生長因子β1（TGF-β1）及單核細(xì)胞趨化蛋白1（MCP-1）的影響，初步探討其作用機制。 方法 本研究采用高脂高糖飼料喂養(yǎng)+單側(cè)腎結(jié)扎+腹腔注射2%鏈脲佐菌素（STZ）造成糖尿病腎病大鼠模型，成模后將其隨機分為模型組，厄貝沙坦組，芪葵顆粒高、中、低劑量組，另設(shè)假手術(shù)組，每組10只，連續(xù)灌胃給藥8周。血糖儀測定大鼠血糖值，酶聯(lián)免疫吸附測定（ELISA）法檢測血清中白細(xì)胞介素6（IL-6）的含量，免疫組化法檢測腎組織中MCP-1、TGF-β1的表達。 結(jié)果 與模型組比較，芪葵顆粒高、中劑量組大鼠血糖顯著降低，差異有統(tǒng)計學(xué)意義（P < 0.05）；與實驗前比較，芪葵顆粒高、中、低劑量組大鼠血糖均顯著降低（P < 0.01或P < 0.05）；與模型組比較，芪葵顆粒高、中、低劑量組大鼠血清中IL-6含量明顯降低（P < 0.01），腎組織中MCP-1、TGF-β1的表達明顯減少（P < 0.01）。 結(jié)論 芪葵顆粒對糖尿病腎病大鼠腎組織具有較好的保護作用，其作用機制可能與其降低血糖、減少IL-6和抑制MCP-1、TGF-β1的表達有關(guān)。

[關(guān)鍵詞] 芪葵顆粒；糖尿病腎??；白細(xì)胞介素6；單核細(xì)胞趨化蛋白1；轉(zhuǎn)化生長因子β1

[中圖分類號] R587；R692.3 [文獻標(biāo)識碼] A [文章編號] 1673-7210（2016）12（b）-0008-04

Effect of Qikui Granules on the expression of TGF-β1 and MCP-1 in kidney of rats with diabetic nephropathy

YANG Chen ZHU Xuanxuan YU Jiangyi ZHANG Di

Pharmacology Laboratory， Affiliated Hospital of Nanjing University of Chinese Medicine， Jiangsu Province， Nanjing 210029， China

[Abstract] Objective To observe the effects of Qikui Granules on the expression of transforming growth factor β1 （TGF-β1） and monocyte chemotactic protein 1 （MCP-1） in kidney of rats with diabetic nephropathy， and to evaluate the mechanism. Methods This experiment adopted the high-sugar-high-fat diet + unilateral renal ligation + intraperitoneal injection of 2% STZ to establish rat models with diabetic nephropathy， then the rats were randomly divided into model group， Irbesartan group， Qikui Granules of high， medium， low dose group， and sham group， with 10 rats in each group. Continuous intragastric administration of 8 weeks was taken. The values of blood glucose in rats were measured by blood glucose meter. The contents of serum interleukin 6 （IL-6） were determined by enzyme linked immunosorbent assay （ELISA）. The expression of MCP-1 and TGF-β1 in kidney was evaluated by immunohistochemistry method. Results Compared with model group， the levels of blood glucose in Qikui Granules of high， medium dose groups were decreased （P < 0.05）. The levels of blood glucose in Qikui Granules of high， medium， low dose groups were significantly lower than those before experiment （P < 0.01 or P < 0.05）. Compared with model group， the contents of IL-6 in Qikui Granules of high， medium， low dose groups were significantly decreased （P < 0.01）， the expression of MCP-1 and TGF-β1 in kidney of Qikui Granules of high， medium， low dose groups was significantly reduced （P < 0.01）. Conclusion Qikui Granules have better protective effects for the renal tissue of rats with diabetic nephropathy. Its mechanism may be related to the decrease of blood glucose and IL-6， the inhibition of the expression of MCP-1 and TGF-β1.