MicroRNA-200b調(diào)節(jié)口腔鱗癌E-鈣黏著蛋白的研究

張軼　王寅　吳劍　李龍江

[摘要] 目的 通過(guò)口腔鱗癌細(xì)胞中過(guò)表達(dá)microRNA-200b，檢測(cè)口腔鱗癌細(xì)胞E-鈣黏著蛋白表達(dá)是否有所上調(diào);并檢測(cè)E-鈣黏著蛋白表達(dá)上調(diào)對(duì)口腔鱗癌細(xì)胞生物學(xué)特性的影響。 方法 通過(guò)目的基因過(guò)表達(dá)慢病毒載體構(gòu)建（has-miR 200b-LV），轉(zhuǎn)染舌癌細(xì)胞株Tca8113，檢測(cè)轉(zhuǎn)染后舌癌細(xì)胞株Tca8113的E-鈣黏著蛋白的表達(dá)及轉(zhuǎn)染后舌癌細(xì)胞株Tca8113的侵襲和轉(zhuǎn)移情況。 結(jié)果 實(shí)驗(yàn)組通過(guò)Real-time PCR數(shù)值分析顯示miR-200b基因表達(dá)定量分析平均值比較，組間差異有統(tǒng)計(jì)學(xué)意義（P<0.05）;轉(zhuǎn)染后的Tca8113細(xì)胞，通過(guò)Real-time PCR數(shù)值分析顯示實(shí)驗(yàn)組中E-cadherin基因表達(dá)定量分析平均值比較，組間差異有統(tǒng)計(jì)學(xué)意義（P<0.05）;Transwell侵襲實(shí)驗(yàn)中，空白組和對(duì)照組穿膜細(xì)胞數(shù)分別為（29.3±4.5）和（20.2±3.3），與實(shí)驗(yàn)組（4.3±2.1）比較，差異有統(tǒng)計(jì)學(xué)意義（P<0.05）;生長(zhǎng)曲線顯示microRNA-200b過(guò)表達(dá)病毒感染的腫瘤細(xì)胞生長(zhǎng)速度明顯減慢。 結(jié)論 microRNA-200b的表達(dá)可以調(diào)控E-鈣黏著蛋白的表達(dá)，E-鈣黏著蛋白的高表達(dá)可以抑制腫瘤的侵襲和轉(zhuǎn)移。

[關(guān)鍵詞] microRNA-200b;E-鈣黏著蛋白;侵襲;轉(zhuǎn)移

[中圖分類號(hào)] R739.85 ? ? ? ? ?[文獻(xiàn)標(biāo)識(shí)碼] A ? ? ? ? ?[文章編號(hào)] 1674-0742（2020）10（a）-0033-03

microRNA-200b Regulating E-cadherin in Oral Squamous Cell Carcinoma

ZHANG Yi1，2， WANG Yin2， WU Jian2， LI Long-jiang2

1.Department of Stomatology， Zhongshan Hospital， Xiamen University， Xiamen， Fujian Province， 361004 China; 2.Surgery of Head and Neck Oncology， West China Hospital of Stomatology， Sichuan University， Chengdu， Sichuan Province， 610041 China

[Abstract] Objective To detect whether the expression of E-cadherin in oral squamous cell carcinoma cells is up-regulated by overexpressing microRNA-200b in oral squamous cell carcinoma cells; and to detect the effect of up-regulation of E-cadherin expression on the biological characteristics of oral squamous cell carcinoma cells influences. Methods The target gene overexpression lentiviral vector （has-miR 200b-LV） was constructed and transfected into the tongue cancer cell line Tca8113. The expression of E-cadherin in the tongue cancer cell line Tca8113 after transfection and the tongue cancer cell line after transfection were detected. Results The real-time PCR numerical analysis of the experimental group showed that the average value of miR-200b gene expression quantitative was compared， and the difference between the groups was statistically significant （P<0.05）; the transfected Tca8113 cells passed Real-time Time PCR numerical analysis showed that the average value of E-cadherin gene expression quantitative analysis in the experimental group was （compared）， and the difference between the groups was statistically significant （P<0.05）; in the Transwell invasion experiment， the blank group and the control group had permeabilized cells numbers were （29.3±4.5） and （20.2±3.3）， which were statistically different from the experimental group （4.3±2.1） （P<0.05）; the growth curve showed the growth rate of microRNA-200b overexpression virus-infected tumor cells was significantly slowed down. Conclusion The expression of microRNA-200b can regulate the expression of E-cadherin， and the high expression of E-cadherin can inhibit tumor invasion and metastasis.

綜上所述，microRNA-200b的表達(dá)可以明顯上調(diào)E-鈣黏著蛋白的表達(dá)， E-鈣黏著蛋白的高表達(dá)可以抑制腫瘤的侵襲和轉(zhuǎn)移。

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（收稿日期：2020-07-05）