HSP70表達水平在啟動正常分娩過程中的作用及機制研究
2021-08-18 21:29:39賀琴盛明燕虞燕爾
中國現(xiàn)代醫(yī)生 2021年19期
賀琴 盛明燕 虞燕爾
[關(guān)鍵詞] HSPA1A;HSPA8;ERS2;正常分娩;HSP70
[中圖分類號] R714.3? ? ? ? ? [文獻標識碼] A? ? ? ? ? [文章編號] 1673-9701（2021）19-0044-05
Study on the effect and mechanism of HSP70 expression level in initiating normal delivery
HE Qin? ?SHENG Mingyan YU Yan′er
Department of Obstetrics and Gynecology， Beilun Branch， the First Affiliated Hospital， Zhejiang University， Ningbo 315800， China
[Abstract] Objective To explore the role and mechanism of HSP70 expression level in initiating normal delivery. Methods HSPA1A and HSPA8 recombinant plasmids and Si-HSPA8， Si-HSPA1A were transfected into WISH cells. The expression levels of IL-8， FAS， BAX， and ESR2 in the cells were detected by ELISA. WISH cells were treated with phosphorylation inhibitor PD98059. The expression of 17 bβ-Estradiol， ERK1/2， p-ERK1/2， ERS2， and P-ERS2 in the cells was detected by qRT-PCR and Western-blot. Results 48 h after transfection， the HSPA1A and HSPA8 in the pcDNA3.1（-）-HSPA1A and pcDNA3.1（-）-HSPA8 recombinant plasmid groups increased， and the HSPA1A and HSPA8 induced by siRNA interference decreased. The expressions of BAX， FAS， ESR2 in the HSPA1A group， and HSPA8 group were lower than those in the NC group. The expression of IL-8 in the HSPA1A group and HSPA8 group was higher than that in the NC group （P<0.05）. The BAX， FAS， ESR2 in the Si-HSPA1A， and Si-HSPA8 groups were higher than in the Si-NC group， and the expression of IL-8 was lower than in the Si-NC group （P<0.05）. Compared with the control group，the 17β-Estradiol concentration increased when HSPA1A and HSPA8 were overexpressed. After the HSPA1A and HSPA8 genes were knocked out，there was no difference in 17β-Estradiol concentration compared with the Si-NC group（P>0.05）. When ERK1/2（PD98059） inhibitor treated WISH cells， the 17β-Estradiol concentration was significantly higher than that of the control group （P<0.05）. Compared with the control group，the ERK1/2 and p-ERK1/2 in the cells decreased significantly when HSPA1A and HSPA8 were overexpressed， while the levels of ERK1/2 and p-ERK1/2 in cells were significantly higher when HSPA1A and HSPA8 were knocked down. The expression of ESR2 and p-ESR2 in WISH cells treated with PD 98059 was significantly lower than that in the control group. Conclusion HSP70 may regμLate the activation of ESR2 through ERK1/2， which may stimμLate the body′s estrogen secretion to ensure a smooth delivery process.
