五葉地錦果實多糖、花色苷抗氧化、抗腫瘤活性研究

柴軍紅　何婷婷　趙楠　金志民

摘要：采用體外抗氧化體系研究五葉地錦（Parthenocissus quinquefolia）果實多糖、花色苷對超氧自由基、羥基自由基、DPPH自由基的清除能力，采用MTT法研究果實中花色苷和多糖對兩種肝癌細胞（HepG2，SMMC-7221）的抑制作用。結(jié)果表明，多糖和花色苷濃度與肝癌細胞的抑制率具有劑量依賴關(guān)系，當(dāng)花色苷濃度達到0.312 5 mg/mL時，對兩種肝癌細胞的抑制率分別為37.89%±1.74%、53.57%±1.72%;當(dāng)多糖提取液濃度達到0.312 5 mg/mL時，對兩種肝癌細胞的抑制率分別為79.59%±0.52%、58.85%±1.12%;多糖、花色苷濃度在0.80 mg/mL時對超氧自由基的清除率分別達到74.21%±2.48%、90.06%±0.86%;對羥基自由基的清除率達到75.87%±1.61%、68.60%±1.52%;對DPPH自由基的清除率達到91.41%±0.74%、90.73%±0.44%。另外，羥基自由基、超氧自由基清除能力與抗腫瘤之間顯示相關(guān)性，與DPPH相關(guān)性較差。五葉地錦果實中提取的多糖和花色苷對肝癌細胞具有一定抑制作用，同時表現(xiàn)出好的抗氧化能力，且兩者之間存在明顯相關(guān)性，具有開發(fā)潛力。

關(guān)鍵詞：五葉地錦（Parthenocissus quinquefolia）果實;花色苷;多糖;抗氧化;MTT

中圖分類號：R965.1;R735.7? ? ? ? ?文獻標(biāo)識碼：A

文章編號：0439-8114（2019）15-0097-05

DOI：10.14088/j.cnki.issn0439-8114.2019.15.023? ? ? ? ? ?開放科學(xué)（資源服務(wù)）標(biāo)識碼（OSID）：

Study on antioxidant and antitumor activity of polysaccharide

and anthocyanin from Parthenocissus quinquefolia fruit

CHAI Jun-hong，HE Ting-ting，ZHAO Nan，JIN Zhi-min

（Mudanjiang Normal University，Mudanjiang 157011，Heilongjiang，China）

Abstract： In vitro the antioxidant activity of polysaccharide and anthocyanin on Parthenocissus quinquefolia fruit was discussed. Study on the scavenging ability of polysaccharide and anthocyanin with superoxide free radical， hydroxyl radical and DPPH free radical. Using MTT method to study the anthocyanins and polysaccharides in inhibition of 2 kinds of hepatoma cells（HepG2， SMMC-7221）. The results showed that the polysaccharide and anthocyanin concentration has a dose-dependent relationship with the HCC inhibitory rate， when the anthocyanin concentration reached 0.312 5 mg/mL， the inhibition rate of two HCC was 37.89%±1.74%， 53.57%±1.72%; when the polysaccharide concentration reached 0.312 5 mg/mL， the inhibition rate of two HCC cells was 79.59%±0.52%， 58.85%±1.12%. The polysaccharide or anthocyanin was 0.80 mg/mL， the scavenging rate of superoxide radicals was 74.21%±2.48%、90.06%±0.86%， the hydroxyl radical was 75.87%±1.61%、68.60%±1.52%， the scavenging rate of DPPH was 91.41%±0.74%、90.73%±0.44%. Furthermore，the scavenging ability of hydroxyl radical has a correlation with antitumor. The superoxide radicals also showed certain correlation， and the correlation of DPPH was poor. Parthenocissus quinquefolia fruit polysaccharide and anthocyanin has certain inhibitory effect on HCC and radical， there is a clear correlation with antioxidant and antitumor;it has the development potential.