基于轉(zhuǎn)錄組測序技術(shù)初步篩選內(nèi)蒙古特稟體質(zhì)人群的差異表達(dá)基因

薛滋平 康陸佼 張亞軍

[摘要] 目的 運用轉(zhuǎn)錄組測序技術(shù)研究內(nèi)蒙古地區(qū)特稟體質(zhì)與平和體質(zhì)人群差異基因的表達(dá)，以期從分子水平探討中醫(yī)特稟體質(zhì)的成因、特征及相關(guān)過敏性疾病的發(fā)生機制。 方法 對2015年9月～2016年9月內(nèi)蒙古醫(yī)科大學(xué)第一附屬醫(yī)院、通遼市醫(yī)院以及巴彥淖爾市醫(yī)院體檢中心的受檢者進(jìn)行流行病學(xué)調(diào)查，篩選祖輩三代均生活在內(nèi)蒙古地區(qū)的特稟體質(zhì)和平和體質(zhì)人群為研究對象，各取10例受試者外周血進(jìn)行轉(zhuǎn)錄組測序，對所得高表達(dá)基因進(jìn)行qPCR驗證來反映部分差異表達(dá)基因及其相關(guān)通路與特稟體質(zhì)的相關(guān)性。 結(jié)果 與平和體質(zhì)比較，特稟體質(zhì)的所有下調(diào)基因中，HLA-DRB1、HLA-DRB5和HLA-DQA2均富集在哮喘通路上;Toll樣受體2（TLR2）差異表達(dá)基因富集在與特稟體質(zhì)關(guān)系密切的TLR信號通路上。qPCR驗證發(fā)現(xiàn)特稟體質(zhì)CPNE3基因相對表達(dá)量明顯高于平和體質(zhì)，差異有統(tǒng)計學(xué)意義（P < 0.05）。 結(jié)論 HLAⅡ類基因與特稟體質(zhì)密切相關(guān)且在特稟體質(zhì)中差異表達(dá);基因TLR2參與的TLR信號通路體現(xiàn)了過敏性疾病的信號傳導(dǎo)機制可能與特稟體質(zhì)密切相關(guān);CPNE3基因表達(dá)在特稟體質(zhì)和平和體質(zhì)中存在差異，提示兩種體質(zhì)間基因表達(dá)可能存在一定差異，但仍需進(jìn)一步研究。

[關(guān)鍵詞] 轉(zhuǎn)錄組測序技術(shù);特稟體質(zhì);表達(dá)基因

[中圖分類號] R22 [文獻(xiàn)標(biāo)識碼] A [文章編號] 1673-7210（2018）12（b）-0008-05

[Abstract] Objective To study the differential gene expression in the special constitution and the moderate constitution of Inner Mongolia by using the transcriptome sequencing technology， in order to explore the genesis， characteristics and related mechanisms of allergic diseases from the molecular level. Methods From September 2015 to September 2016， the Affiliated Hospital of Inner Mongolia Medical University， Tongliao City Hospital and the Physical Examination Center of Bayanur Hospital were investigated by epidemiology. The special constitution and the moderate constitution of the three generations of ancestors living in Inner Mongolia were selected as the research objects， the peripheral blood of 10 subjects were taken for transcriptome sequencing. Group sequencing and qPCR were used to verify the correlation between some differentially expressed genes and their related pathways and special constitution. Results Compared with moderate constitution， among all down-regulated genes in special constitution， HLA-DRB1， HLA-DRB5 and HLA-DQA2 were enriched in the asthma pathway; Toll like receptor 2 （TLR2） differentially expressed genes were enriched in the TLR signaling pathway， which was closely related to the special constitution. The qPCR test showed that the relative expression of CPNE3 genes in the special constitution was higher than that in the moderate constitution， the difference was statistically significant （P < 0.05）. Conclusion HLA class Ⅱ genes are closely related to special constitution and differentially express in the special constitution; TLR signaling pathway involved by gene TLR2 reflects that the signal transduction mechanism of allergic diseases may be closely related to special constitution; CPNE3 gene expression differences in special constitution and moderate constitution， indicating that there may be some differences in the expression of the two constitutions， but further studies are needed.