SCFAs對DCAPCs中GH、PRL合成和分泌的影響及其初步機(jī)制研究中期報(bào)告

王建發(fā) 付守鵬 王瑋 柳巨雄 雷連成

摘 要：該研究采用RT-PCR、ELISA、液體閃爍測量技術(shù)、western blot等方法研究了SCFAs對DCAPCs中cAMP/PKA/CREB信號通路的影響，以及對GH、PRL表達(dá)和分泌的影響，并初步認(rèn)識了SCFAs調(diào)節(jié)DCAPCs中GH、PRL表達(dá)和分泌的分子機(jī)制。SCFAs可以通過與DCAPCs細(xì)胞膜上的GPR41/43相結(jié)合，激活Gαi蛋白，抑制AC的酶活性，降低胞內(nèi)cAMP含量，從而降低PKA活性和CREB磷酸化水平，最終以直接或間接的方式下調(diào)了GH和PRL的合成和分泌。此外，SCFAs可以降低DCAPCs的細(xì)胞活性，這也可能是導(dǎo)致GH和PRL分泌量減少的原因之一。

關(guān)鍵詞：斷鏈脂肪酸 奶牛 生長激素 催乳素 G蛋白

The Effects and Molecular Mechanisms of Short-Chain Fatty Acids on GH and PRL Synthesis and Secretion in DCAPCs

Wang Jianfa Fu Shoupeng Wang Wei Liu Juxiong Lei Liancheng

（Jilin University）

Abstract：Short-chain fatty acids （SCFAs） play a key role in altering carbohydrate and lipid metabolism， influence endocrine pancreas activity， and as a precursor of ruminant milk fat. However， the effect and detailed mechanisms by which SCFAs mediate bovine growth hormone （GH） and prolactin （PRL） gene transcription remain unclear. In this study， we detected the effects of SCFAs （acetate， propionate， and butyrate） on the activity of the cAMP/PKA/CREB signaling pathway， GH， PRL， and Pit-1 gene transcription in dairy cow anterior pituitary cells （DCAPCs）.The results showed that SCFAs decreased intracellular cAMP levels and a subsequent reduction in PKA activity.Inhibition of PKA activity decreased CREB phosphorylation，thereby inhibiting GH and PRL gene transcription.Furthermore，PTX blocked SCFAs-inhibited cAMP/PKA/CREB signaling pathway.These data showed that the inhibition of GH and PRL gene transcription induced by SCFAs is mediated by Gi activation and that propionate is more potent than acetate and butyrate in inhibiting GH and PRL gene transcription. In conclusion，this study identifies a biochemical mechanism for the regulation of SCFAs on bovine GH and PRL gene transcription in DCAPCs，which may serve as one of the factors that regulate pituitary function in accordance with dietary intake.

Key Words：Short-chain fatty acids；Dairy cow；Growth hormone；Prolactin；G protein

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